ZRB1533

Anti-DYNC1I1 Antibody, clone 1F11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Anti-SV40 T Antigen Antibody, clone 1F11 ZooMAb^® Mouse Monoclonal, Cytoplasmic dynein 1 intermediate chain 1, Cytoplasmic dynein intermediate chain 1, DH IC-1, Dynein intermediate chain 1 cytosolic
• NACRES: NA.43
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 1F11, recombinant monoclonal
• Application: IF, IHC, WB, affinity binding assay
• Citations: -

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1F11, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 72.96 kDa, observed mol wt ~73 kDa
• purified by: using Protein A
• species reactivity: mouse, human
• species reactivity (predicted by homology): monkey, feline, porcine, canine, bovine
• packaging: antibody small pack of 25
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): affinity binding assay: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_004402
• UniProt accession no.: O14576
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... DYNC1I1(1780)

Beschreibung

General description

Cytoplasmic dynein 1 intermediate chain 1 (UniProt: O14576; also known as Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1) is encoded by the DYNC1I1 (also known as DNCI1, DNCIC1) gene (Gene ID: 1780) in human. DYNC1I1 is a widely expressed, homodimeric protein of the dynein intermediate chain family with seven WD repeats. It acts as one of the several non-catalytic accessory components of the cytoplasmic dynein 1 complex that is involved in linking dynein to cargos and to adapter proteins that regulate dynein function. It is located in several cellular components, including kinetochore, recycling endosomes, and spindle pole. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its N-terminal amino acid sequence (aa 2-123). It may also play a role in mediating the interaction of cytoplasmic dynein with me

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal region of human Cytoplasmic dynein 1 intermediate chain 1 (DYNC1I1).

Application

Quality Control Testing

Evaluated by Western Blotting in Mouse brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DYNC1I1 in Mouse brain tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected DYNC1I1 in Human brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected DYNC1I1 in Human cerebral cortex tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound DYNC1I1 peptide with a KD of 5.7 x 10-7 in an affinity binding assay.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected DYNC1I1 in Human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone 1F11 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Cytoplasmic dynein 1 intermediate chain 1 (DYNC1I1). It targets an epitope within 23 amino acids from the N-terminal region.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Was ist los?: WGK 1
• Lagerklasse: 11 - Combustible Solids