ZRB1501

Anti-PEDF (SERPIN F1) Antibody, clone 2C16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Cell proliferation-inducing gene 35 protein, EPC-1, PEDF, Pigment epithelium-derived factor
• NACRES: NA.43
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 2C16, monoclonal, recombinant monoclonal
• Application: IHC (p), WB, affinity binding assay
• Citations: -

Eigenschaften

• biological source: rabbit (recombinant)
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2C16, monoclonal, recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 46.31 kDa, observed mol wt ~46 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): affinity binding assay: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG
• UniProt accession no.: P36955
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... SERPINF1(5176)

Beschreibung

General description

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Pigment epithelium-derived factor (UniProt: P36955; also known as PEDF, Cell proliferation-inducing gene 35 protein, EPC-1, Serpin F1) is encoded by the SERPINF1 (also known as PEDF) gene (Gene ID: 5176) in human. Serpin F1 is a monomeric secreted protein that is synthesized with a signal peptide (aa 1-19) that is subsequently cleaved off to produce the mature form. Serpin F1 is synthesized by multiple cells, including retinal pigment epithelium, fibroblasts, mammary epithelium, adipocytes, hepatocytes and osteoblasts. It is enriched in retinal pigment epithelial cells and blood plasma. It displays neurotrophic activity and induces extensive neuronal differentiation in retinoblastoma cells. Its N-terminal (aa 44-121) displays neurite outgrowth-inducing activity while the C-terminal exposed loop (aa 382-418) is reported to be essential for its serpin activity. Serpin F1 also serves as a potent inhibitor of angiogenesis. Serpin F1 contains three beta sheets, 8 10 alpha helices, and a C terminal reactive center loop. In the cell, serpin F1 undergoes phosphorylation at Ser227 by protein kinase A that increases its anti-angiogenic activity and extracellular serpin F1 can be phosphorylated on serine 24 and serine 114 by casein kinase 2 that promotes its neurotrophic activity. Mutations in SERPINF1 gene are shown to cause Osteogenesis imperfecta 6 that is characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Immunogen

GST/His-tagged recombinant fragment corresponding to 152 amino acids from the C-terminal half of human Pigment epithelium-derived factor (PEDF).

Application

Affinity Binding Assay: A representative lot of this antibody bound human recombinant PEDF with a KD of 8.1 x 10-9 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PEDF (SERPIN F1) in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-PEDF (SERPIN F1), clone 2C16 ZooMAb, Cat. No. ZRB1501, is a recombinant Rabbit monoclonal antibody that specifically targets PEDF and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.

Biochem/physiol Actions

Clone 2C16 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Pigment epithelium-derived factor (PEDF).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable