ZRB1355

Anti-Cardiac Troponin I Antibody, clone 1F23, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Troponin I
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 1F23, monoclonal, recombinant monoclonal
• Application: ICC, IF, IHC, WB
• Citations: 1

Eigenschaften

• biological source: rabbit (recombinant)
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1F23, monoclonal, recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 24.0 kDa, observed mol wt ~26 kDa
• species reactivity: human, rat, mouse
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG
• UniProt accession no.: P19429
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... TNNI1(7135)

Beschreibung

General description

Troponin I, cardiac muscle (UniProt: P19429; also known as Cardiac troponin I) is encoded by the TNNI3 (also known as TNNC1) gene (Gene ID: 7137) in human. Troponin is a complex that regulates the contraction of skeletal muscle. It consists of 3 subunits known as C, T, and I, which are located periodically along the thin filament of the myofibrils. Troponins are found in skeletal and cardiac muscle, but not in smooth muscle. Troponin C (TnC) binds calcium ions, Troponin I is the inhibitory subunit of troponin that inhibits actomyosin ATPase and Troponin T binds to tropomyosin. It also confers calcium-sensitivity to actomyosin ATPase. Amino acids region 32-79 of Troponin I is involved in binding to TnC and region 129-149 is involved in binding to both TnC and actin. Cardiac muscle Troponin I has 31 amino acid residues at its N-terminal that are not present in the skeletal muscle form. Hence, cardiac specificity of this isoform is shown to offer accuracy in diagnosis in acute or chronic skeletal muscle injury. Mutation in TNNI3 gene are reported to result in multiple types of cardiomyopathies that can result in premature death. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, H-C., and Fajer, PG (1998). Biochemistry 37(19); 6628-6635; Skeik, N., and Patel, DC (2007). Int. J. Angiol. 16(2); 53-58).

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Immunogen

KLH-conjugated liner peptide corresponding to 14 amino acids from the C-terminal region of human Cardiac Troponin I.

Application

Anti-Cardiac Troponin I, clone 1F23 ZooMAb, Cat. No. ZRB1355, a Rabbit monoclonal antibody that targets Cardiac Troponin I and is tested in Immunocytochemisty, Immunofluorescence, Immunohistochemistry and Western Blotting.

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Cardiac Troponin I in human heart tissue lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cardiac Troponin I in AC16 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Cardiac Troponin I in human heart and rat heart tissue sections.

Immunofluorescence Analysis: A 1:1000 dilution from a representative lot detected Cardiac Troponin I in human heart muscle tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone 1F23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects cardiac troponin I. It targets an epitope within 14 amino acids from the C-terminal region.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable