ZRB1267

Anti-CGRP-1 Antibody, clone 2E18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Anti-CALC, Anti-CCALCI, Anti-CGRP-I
• NACRES: NA.43
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 2E18, monoclonal, recombinant monoclonal
• Application: ICC, IHC (p), WB, affinity binding assay
• Citations: -

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2E18, monoclonal, recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt ~13.90 kDa, observed mol wt ~14 kDa
• species reactivity: human, mouse, rat
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG
• UniProt accession no.: P06881
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Beschreibung

General description

Calcitonin gene-related peptide 1 (UniProt: P06881; also known as Alpha-type CGRP, Calcitonin gene-related peptide I, CGRP-I) is encoded by the CALCA (also known as CALC1) gene (Gene ID: 746) in human. CGRP, a 37 amino acid neuropeptide, is a highly potent and long-acting vasodilator with protective effects in the cardiovascular system. Two forms of CGRP have been described -CGRP-I and CGRP-II (also known as aCGRP and bCGRP) that are synthesized from two distinct genes. CGRP I is the primary neuronal form that is found in the CNS and in the peripheral nervous system and displays higher immunoreactivity than CGRP II. CGRP I is synthesized with a signal peptide (aa 1-25) and two peptides (aa 26-80 and 125-128) that are cleaved off to generate biologically active form (aa 83-119). Following synthesis, it is stored in large, dense-core vesicles within the sensory nerve terminal and is released following neuronal depolarization via calcium-dependent exocytosis. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Russell, FA., et al. (2014). Physiol. Rev. 94(4): 1099-1142).

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from human active Calcitonin gene-related peptide 1 (C-terminal region of the uncleaved peptide)

Application

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Calcitonin Gene-Related Peptide 1 (CGRP-1) in MCF-7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CGRP-1 in PC12 and K562 cells and in Human and mouse liver lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CGRP-1 in human cerebral cortex and human DRG tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound CGRP-1 peptide with a KD of 1.9 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CGRP-1 in MCF7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CGRP-1, clone 2E18 ZooMAb^®, Cat. No. ZRB1267, is a recombinant Rabbit monoclonal antibody that specifically targets CGRP-1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Biochem/physiol Actions

Clone 2E18 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Calcitonin gene-related peptide 1. It targets an epitope within 22 amino acids from the active form of CGRP1.

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable