ZRB1145

Anti-HSP60 Antibody, clone 1N3, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 1N3, recombinant monoclonal
• Application: ICC, IHC (p), WB
• Citations: -

Eigenschaften

• biological source: rabbit (recombinant)
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1N3, recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 61.01 kDa, observed mol wt ~61 kDa
• species reactivity: human, rat, mouse
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable (peptide)
• isotype: IgG
• UniProt accession no.: P10809
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... HSPD1(3329)

Beschreibung

General description

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

60 kDa heat shock protein, mitochondrial (UniProt: P10809; also known as 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein) is encoded by the HSPD1 (also known as HSP60) gene (Gene ID: 3329) in human. Hsp60 is a homoheptameric, highly conserved molecular chaperone present in the mitochondrial matrix where along with Hsp10 it helps in protein folding. It also prevents misfolding of proteins and promotes refolding. It is transcribed by a nuclear gene, translated in the cytosol and the newly translated protein is imported into mitochondria. Its mitochondrial import signal (MIS) is localized in the 26 amino acids at the N-terminus. Following its import, the MIS peptide is cleaved off to generate the mature protein. Its mitochondrial import depends on the mitochondrial membrane potential. Under certain pathological conditions, such as cancer and chronic inflammatory diseases, Hsp60 can also accumulate in the cytosol via a mitochondrial export mechanism. Two isoforms of Hsp60 have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Vilasi, S., et al. (2014). PLoS One. 9(5); e97657).

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Immunogen

GST/HIs-tagged recombinant fragment corresponding to 65 amino acids from the C-terminal half of human Heat Shock Protein 60, isoform 1.

Application

Anti-HSP60, clone 1N3 ZooMAb , Cat. No. ZRB1145, is a highly specific rabbit recombinant monoclonal antibody, that targets Heat Shock Protein 60 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected HSP60 in C6 and NIH/3T3 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected HSP60 in human kidney and mouse kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HSP60in HUVEC and HeLa cells.

Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

Biochem/physiol Actions

Clone 1N3 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Heat shock protein 60. It targets an epitope within 65 amino acids from the C-terminal half.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable