X4126

Xylencyanol FF

BioReagent, Molecular Biology

• Synonym(e): Cyanol FF, Säureblau 147, XC
• Empirische Formel (Hill-System): C₂₅H₂₇N₂NaO₆S₂
• CAS-Nummer: 2650-17-1
• Molekulargewicht: 538.61
• UNSPSC Code: 12171500
• NACRES: NA.52
• PubChem Substance ID: 57654732
• EC Number: 220-167-5
• Colour Index Number: 42135
• MDL number: MFCD00019481

Eigenschaften

• grade: Molecular Biology
• Quality Segment: 200
• product line: BioReagent
• form: powder
• technique(s): nucleic acid detection: suitable
• solubility: H₂O: complete 1 mg/mL, clear, blue
• suitability: suitable for nucleic acid staining
• storage temp.: room temp
• SMILES string: [Na+].CCNc1ccc(cc1C)C(=C2/C=CC(=N\CC)\C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O
• InChI: 1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23-;
• InChI key: VVLFAAMTGMGYBS-KRQUPCAFSA-M

Beschreibung

General description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Xylene Cyanol FF is used as a:

• histological stain and as tracker dye in DNA sequencing and electrophoresis[1][2]
• gel-loading dye
• formamide RNA loading dye
• tracking dye in native polyacrylamide gel electrophoresis (PAGE) loading buffer

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Preparation Note

A 6x Gel Loading Buffer can be made using standard recipes, including 0.05% xylene cyanol.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves