T9950

Tau-352 human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE), lyophilized powder

• Synonym(e): Human Tau Protein
• UNSPSC Code: 12352200
• NACRES: NA.32
• MDL number: MFCD07785730
• Form: lyophilized powder
• Assay: ≥90% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in E. coli
• Mol wt: 36.8 kDa

Eigenschaften

• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥90% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 36.8 kDa
• UniProt accession no.: P10636
• application(s): cell analysis
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... MAPT(4137)

Beschreibung

Application

Tau-352 human is a Tau isoform, variant 0N3R which means it consists of 3 microtubule binding repeats (R) and no amino terminal inserts (N). The product can be utilized for systemic identification of Phosphorylation sites in microtubule-associated protein tau by using checkpoint kinases Chk1 and Chk2 in vitro.[1]

Biochem/physiol Actions

Isoform of Tau, variant 0N3R, having 3 microtubule binding repeats (R) and no amino terminal inserts (N).

Tau-383 human belongs to neuronal microtubule-associated protein family that stimulates the microtubule assembly and stability as well as facilitates hyperphosphorylation-induced self-assembly into filaments.[2] Tau protein along with src can upregulate tyrosine kinase activity. Inhibition of Src kinase influences the action of tau on actin rearrangements which in turn impacts the growth-factor-induced actin remodeling.[3]

Preparation Note

Lyophilized from MES, pH 6.8, containing NaCl and EGTA. When reconstituted in water to a protein concentration of 1 mg/mL, the resulting buffer will have ~50 mM MES, pH 6.8, 100 mM NaCl, and 0.5 mM EGTA.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)