Fortfahren mit
T9943
Trizma® Tris-Kristalle
BioPerformance Certified, pH 7.0, average Mw 154.8
Synonym(e):
Tris-Kristalle
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Über diesen Artikel
NACRES:
NA.25
UNSPSC Code:
12161700
grade
BioPerformance Certified
Quality Segment
form
crystalline powder
mol wt
average Mw 154.8
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, NICKase, none detected, endotoxin, tested, total aerobic microbial count, tested, ≤0.05 A290, ≤0.5% water (Karl Fischer), ≤5 ppm Heavy metals (as Pb)
pH
6.70 (37 °C, 0.1 M)(lit.), 7.00 (25 °C, 0.1 M)(lit.), 7.0, 7.55 (5 °C, 0.1 M)(lit.)
useful pH range
7.0-9.0
application(s)
diagnostic assay manufacturing
Application
Das Produkt wird in Zellkultur getestet. Es wird außerdem auf niedrige Endotoxinwerte getestet und auf die Abwesenheit von Nukleasen und Proteasen analysiert.
Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 °C. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
Packaging
In convenient foil pouches
Preparation Note
Trizma-Lösungen können autoklaviert werden.
Other Notes
Bestimmte pH-Elektroden liefern mit Tris-Puffern keine genauen Ergebnisse.
This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklasse
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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