T1428

apo-Transferrin bovine

BioReagent, suitable for cell culture, ≥98%

• Synonym(e): Human serotransferrin
• CAS-Nummer: 11096-37-0
• UNSPSC Code: 12352202
• EC Number: 234-318-8
• NACRES: NA.83
• MDL number: MFCD00130536
• eCl@ss: 34058011
• Form: powder
• Assay: ≥98%
• Biological source: bovine

Eigenschaften

• biological source: bovine
• Quality Segment: 200
• product line: BioReagent
• assay: ≥98%
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: iron, essentially free
• solubility: H₂O: 10 mg/mL
• UniProt accession no.: Q29443
• storage temp.: 2-8°C
• Gene Information: bovine ... TF(280705)

Beschreibung

General description

apo-Transferrin is an iron-free form of transferrin that is present in circulation.

Application

apo-Transferrin bovine has been used as a component of:

• basal medium for culturing bovine mammary epithelial cells
• minimum essential medium α (MEM-α) for spermatogonial stem cells (SSCs) culture
• induction medium for mesenchymal stem cell culture

All cells require iron for the proper uptake of oxygen from their environment. Transferrin, a protein isolated from serum, causes the uptake and transport of iron from culture medium to the cells. This allows proper oxygen uptake and also stimulates growth-related enzyme activity. Iron-saturated transferrin (holo transferrin) is continually recycled after releasing iron and becomes iron-deficient transferrin (apo-transferrin). Choosing which form to use depends upon the culture conditions. In media such as Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12 (DMEM/F12) that contain high levels of iron salt, using apo-transferrin is preferred.

Biochem/physiol Actions

apo-Transferrin (apoTf) aids in preventing iron accumulation in tissues, especially in non-transfusion dependent β-thalassemia.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)