SRP6505

G6PD human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• Synonym(e): Glucose-6-phosphate 1-dehydrogenase, Zwf
• NACRES: NA.32
• UNSPSC Code: 12352204
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• Form: liquid
• Mol wt: 61.4 kDa (515 aa, 1- 515 aa)

Eigenschaften

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥95% (SDS-PAGE)
• form: liquid
• mol wt: 61.4 kDa (515 aa, 1- 515 aa)
• packaging: pkg of 100 μg
• concentration: 0.5 mg/mL
• UniProt accession no.: P11413
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... G6PD(2539)

Beschreibung

General description

G6PD (glucose-6-phosphate dehydrogenase) gene is mapped to human chromosome Xq28, and spans 16.2kb. One G6PD monomer is composed of 515 amino acids with a predicted molecular weight of 59,256Da. The active enzyme exists as a dimer, and contains an NADP molecule tightly bound to it.

Biochem/physiol Actions

G6PD (glucose-6-phosphate dehydrogenase) enzyme catalyzes the oxidation of glucose-6-phosphate to 6-phosphogluconolactone, reducing NADP to NADPH. It therefore, catalyzes the first step of hexose monophosphate pathway (HMP). As it is responsible for the oxidation of glucose-6-phosphate, and thus, confers protection against oxidative damage in erythrocytes. Its deficiency is highly heterogenous, with around 190 variants being reported. The deficiency of G6PD was discovered while studying hemolytic anemia. G6PD also results in hereditary nonspherocytic hemolytic anemia.

Physical form

0.5 mg/mL solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 mM PMSF, 2 mM EDTA, 2 mM DTT, 200 mM NaCl.

Analysis Note

The biological activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 μmole D-glucose-6-phosphate to 6-phospho-Dgluconate per min in the presence of beta-NADP at pH 7.4 at 25 °C.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319
• pcodes: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Lagerklasse: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable