SRP6437

CD55/DAF human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(e): CR, CROM, TC
• NACRES: NA.32
• UNSPSC Code: 12352200
• Form: lyophilized
• Assay: ≥95% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in HEK 293 cells
• Mol wt: calculated mol wt 35.8 kDa, observed mol wt 50-65 kDa (DTT-reduced. Protein migrates due to glycosylation. Asp 35 is the predicted N-terminal.)

Eigenschaften

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: calculated mol wt 35.8 kDa, observed mol wt 50-65 kDa (DTT-reduced. Protein migrates due to glycosylation. Asp 35 is the predicted N-terminal.)
• packaging: pkg of 10 μg, pkg of 50 μg
• UniProt accession no.: P08174
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CD55(1604)

Beschreibung

General description

CD55 (complement decay-accelerating factor), also known as DAF or decay accelerating factor, is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. CD55 /DAF is a 70 kDa membrane protein that regulates the complement system on the cell surface. This glycoprotein is broadly distributed among hematopoietic and non-hematopoietic cells.

Biochem/physiol Actions

CD55 (complement decay-accelerating factor) prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. It is a determinant for the Cromer blood group system. CD55 is known to bind CD97 via the first short consensus repeats (SCR). It also binds physiologically generated C3 convertases with its second and third SCRs. Binding results in an accelerated “decay”, or dissociation of active C3 convertases, thus blocking the development of C′ attack complexes on non-foreign cells. Finally, viruses and bacteria are also known to utilize multiple SCR sites for infection.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable