SRP6237

SPARC/Osteonectin human

recombinant, expressed in CHO cells, ≥97% (SDS-PAGE)

• Synonym(e): BM-40, ON, Secreted protein acidic and rich in cysteine
• NACRES: NA.32
• UNSPSC Code: 12352200
• Form: lyophilized powder
• Assay: ≥97% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in CHO cells
• Mol wt: 43.7 kDa

Eigenschaften

• biological source: human
• recombinant: expressed in CHO cells
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 43.7 kDa
• packaging: pkg of 10 μg, pkg of 50 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P09486
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... SPARC(6678)

Beschreibung

General description

SPARC/Osteonectin is a secreted, evolutionarily conserved collagen-binding glycoprotein that is involved in a variety of cellular activities. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC/Osteonectin and its related peptides bind to numerous proteins of the extracellular matrix (ECM), affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC/Osteonectin consists of three domains; an N-terminal acidic region that binds calcium ions with low affinity, a module containing two EF-hand motifs that bind calcium with high affinity, and a cysteine-rich follistatin-like domain. Recombinant human SPARC/Osteonectin is a glycoprotein containing 286 amino acids that migrates at an apparent MW of 43.7 kDa by SDSPAGE analysis due to the effect of glycosylation

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.6.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable