SRP6007

NT-3 from mouse

recombinant, expressed in E. coli, ≥97% (SDS-PAGE)

• Synonym(e): HDNF, NGF-2, NT-3, Nerve growth factor-2, Neurotrophic factor
• NACRES: NA.32
• UNSPSC Code: 12352202
• Form: lyophilized
• Assay: ≥97% (SDS-PAGE)
• Biological source: mouse
• Recombinant: expressed in E. coli
• Mol wt: dimer (13.8/27.5 kDa (120/240 amino acids))

Eigenschaften

• biological source: mouse
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE)
• form: lyophilized
• mol wt: dimer (13.8/27.5 kDa (120/240 amino acids))
• packaging: pkg of 10 μg
• impurities: Endotoxin, tested
• NCBI accession no.: P20181
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... NTF3(18205)

Beschreibung

General description

NT-3 (neurotrophin-3) is a member of the family of trophic factors, called neurotrophins. This family also includes NGF (nerve growth factor), BDNF (brain-derived neurotrophic factor), and NT-4. NT-3 is the most abundantly expressed neurotrophin during early development.
Murine NT-3 produced from Escherichia coli contains 119 amino acid and having a molecular mass is 13.6kDa.

Application

NT-3 (neurotrophin-3) has been used for the ex vivo treatment of skin punches obtained from rat and human samples.

Biochem/physiol Actions

Study in mice show that NT-3 (neurotrophin-3), along with BDNF (brain-derived neurotrohpic factor), is essential for inner ear development. In vitro motor, enteric, primary sensory, sympathetic, locus coeruleus, cerebellar and hippocampal neurons are responsive to NT-3. Mice null for NT-3 show significant deficiency in sympathetic and sensory nerve populations. In mice, post birth up-regulation of NT-3 induces a second wave of dopaminergic amacrine cell genesis.

Physical form

Lyophilized from a 0.2 μm filtered solution containing0.1% Trifluoroacetic Acid (TFA)

Preparation Note

Sterile water at 0.1 mg/mLCentrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable