SRP5292

PIP5K1A, active, GST tagged human

PRECISIO^®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): RP11-68I18.9
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected Sf9 cells
• Produktlinie: PRECISIO^®
• Assay: ≥70% (SDS-PAGE)
• Form: buffered aqueous glycerol solution
• Spezifische Aktivität: 947-1281 nmol/min·mg
• Mol-Gew.: ~86 kDa
• NCBI-Hinterlegungsnummer: BC007833
• Anwendung(en): cell analysis
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... PIP5K1A(8394)

Beschreibung

Allgemeine Beschreibung

PIP5K1A or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1 is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells. Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through ′frizzled′ and ′dishevelled′, the latter of which directly interacts with and activated PIP5KI. PIP5K1A-mediated PIP production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes. Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP production and enhances viral replication in primary lymphocytes.

Physikalische Form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Angaben zur Herstellung

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable