SRP5281

SPHK1, active, GST tagged human

PRECISIO^®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): SPHK
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf9 cells
• Produktlinie: PRECISIO^®
• Assay: ≥70% (SDS-PAGE)
• Form: buffered aqueous glycerol solution
• Spezifische Aktivität: 465-592 nmol/min·mg
• Mol-Gew.: ~71 kDa
• NCBI-Hinterlegungsnummer: BC008040
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... SPHK1(8877)

Beschreibung

Allgemeine Beschreibung

SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP) which is a novel lipid messenger with both intracellular and extracellular functions. Various stimuli increase cellular levels of SPP by activation of SPHK1. Competitive inhibitors of SPHK1 block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors. SPHK1/sphingosine-1-phosphate pathway contributes to colon carcinogenesis. SPHK1 plays a key role in the generation and release of proinflammatory mediators triggered by anaphylatoxins from human macrophages and also is involved in the process of immune cell motility.

Physikalische Form

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Angaben zur Herstellung

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable