SRP3323

R-SPONDIN-3 human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonym(e): PWTSR, RSPO3, Roof plate-specific spondin-3
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in CHO cells
• Assay: ≥95% (HPLC); ≥95% (SDS-PAGE)
• Form: lyophilized
• Mol-Gew.: 26.9 kDa
• Verpackung: pkg of 20 μg
• Verunreinigungen: endotoxin, tested
• UniProt-Hinterlegungsnummer: Q9BXY4
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... RSPO3(84870)

Beschreibung

Allgemeine Beschreibung

The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization and resulting accumulation of the intracellular signaling protein, ?-catenin. This activity effectively activates and increases the subsequent nuclear signaling of ?-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/?-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations. Recombinant human R-Spondin-3 is a 26.9 kDa protein consisting of 240 amino acid residues. Due to glycosylation, R-Spondin-3 migrates at an apparent molecular weight of approximately 37.0 kDa by SDS PAGE analysis under reducing conditions.

Biochem./physiol. Wirkung

The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. Recombinant human R-Spondin-3 is a 26.9 kDa protein consisting of 240 amino acid residues.

Physikalische Form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 150 mM NaCl.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable