SRP3284

GASP-1 human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonym(e): GDF-associated serum protein-1
• NACRES: NA.32
• UNSPSC Code: 12352200
• Form: lyophilized
• Assay: ≥95% (HPLC), ≥95% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in CHO cells
• Mol wt: 55.0-66.0 kDa

Eigenschaften

• biological source: human
• recombinant: expressed in CHO cells
• assay: ≥95% (HPLC), ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: 55.0-66.0 kDa
• packaging: pkg of 25 μg
• concentration: 0.0025-0.0040 μg/mL
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: Q8TEU8
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... WFIKKN2(124857)

Beschreibung

General description

Growth and differentiation factor-associated serum protein-1 (GASP-1) is expressed primarily in the ovary, testis, and brain. The GASP-1 gene encodes a 571 amino acid protein that contains a 29 amino acid secretion signal sequence, and multiple identifiable structural features, including a WAP domain, a follistatin/Kazal domain, an immunoglobulin domain, two tandem Kunitz domains, and a netrin domain. Recombinant human GASP-1 is a 542 amino acid protein that migrates at an apparent molecular weight of approximately 55-66kDa by SDS-PAGE analysis under non-reducing conditions.

Biochem/physiol Actions

Growth and differentiation factor-associated serum protein-1 (GASP-1) is a secreted inhibitory TGF-β binding protein that contains multiple protease inhibitor structural domains. It can act as a potent soluble inhibitor of myostatin and GDF-11, but not Activin-A.

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable