SRP2086

p300 (C1135-2414) human

recombinant, expressed in insect cells, ≥70% (SDS-PAGE)

• Synonym(e): KAT3B, RP1-85F18.1, RSTS2, p300
• NACRES: NA.26
• UNSPSC Code: 12352200
• Form: frozen liquid
• Assay: ≥70% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in insect cells
• Mol wt: ~145 kDa

Eigenschaften

• biological source: human
• recombinant: expressed in insect cells
• assay: ≥70% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~145 kDa
• packaging: pkg of 4 μg
• concentration: 350 μg/mL
• color: clear colorless
• NCBI accession no.: NM_001429
• UniProt accession no.: Q09472
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... EP300(2033)

Beschreibung

General description

E1A binding protein p300 (p300) has a central bromodomain and three cysteine- and histidine-rich regions. The carboxy-terminal region interacts with the adenovirus oncoprotein E1A. The gene encoding this protein is localized on human chromosome 22q13.

Biochem/physiol Actions

Human p300 and CBP (CREB binding protein) are highly related transcriptional coactivators. Both proteins have been identified through protein interaction assays. In addition to interacting with a variety of cellular factors and onco-proteins, loss of the wild type CBP alleles in isolated tumors suggests that CBP/p300 might serve as a tumor suppressor. The C-terminus of p300 contains a histone acetyltransferase (HAT) domain expanded from residues 1195 to 1673, an activation domain (residues 1763-1812) that interacts with many transcription factors and an SRC-1 domain further down stream of the activation domain. P300 is able to acetylate histones and many other transcription factors, including p53, E2F, TFIIE, and TFIIF etc. implicating diverse roles of p300 in gene regulation.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable