SRP0723

YSK4 (MAP3K19), Active

PRECISIO^® Kinase, recombinant

• NACRES: NA.77
• UNSPSC Code: 12352202
• Form: frozen liquid
• Assay: 70-95% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells
• Mol wt: ~83 kDa

Eigenschaften

• recombinant: expressed in baculovirus infected Sf9 cells
• assay: 70-95% (SDS-PAGE)
• form: frozen liquid
• specific activity: 30-45 nmol/min·mg (radiometric)
• mol wt: ~83 kDa
• concentration: 0.05 μg/μL
• accession no.: NM_025052
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... YSK4(80122)

Beschreibung

Application

Useful for kinase assay and Western blot.

Biochem/physiol Actions

Recombinant human YSK4 (MAP3K19) (941-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. YSK4 (MAP3K19, RCK) belongs to STE Ser/Thr protein kinase family and STE20 subfamily. The YSK4 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat ⁽¹⁾. The important paralog is MAP3K1 gene. The RCK ligand-binding domains form an octameric gating ring ⁽²⁾ in the large-conductance Ca²⁺-activated K⁺ channels ⁽³⁾. The YSK4 kinase function has not been made clearly yet.

Preparation Note

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable