SRP0410

ALOX15 active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥60% (SDS-PAGE)

• Synonym(e): 15LOX-1, arachidonate 15-lipoxygenase
• NACRES: NA.32
• UNSPSC Code: 12352202

Eigenschaften

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• assay: ≥60% (SDS-PAGE)
• form: aqueous solution
• mol wt: 75 kDa
• packaging: pkg of 20 μg
• NCBI accession no.: NM_001140
• UniProt accession no.: P16050
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... ALOX15(246)

Beschreibung

General description

The gene ALOX15 (arachidonate 15-lipoxygenase) is mapped to human chromosome 17p13. It is mainly present in the epithelial cells in the upper airways, reticulocytes, eosinophils and macrophages. ALOX15 belongs to the dioxygenases family. It is an IL-4 (interleukin 4)/IL-13 target gene.
ALOX-15, also known as 15LOX-1, ω-6 lipoxygenase, or arachidonate 15-lipoxygenase (GenBank Accession No. NM_001140) amino acids 2- 662 (end) with N-terminal FLAG-tag, MW = 75kDa, expressed in Sf9 insect cells via a Baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

ALOX15 (arachidonate 15-lipoxygenase) is involved in homeostatic as well as pathological responses. ALOX15 is responsible for the oxidation of unsaturated fatty acids (at the 15 position) to active hydroperoxy and epoxy metabolites. It is also responsible for the conversion of linoleic acid to anti-tumor 13-S-hydroxyoctadecadienoic acid. ALOX15 is required for IL-4 (interleukin-4)/IL-13-mediated macrophage polarization. In mice, it is a negative regulator of bone mineral density. It is also involved in fatty acid metabolism. It controls MAPK (mitogen activated protein kinase) signaling in human airway epithelial cells using phosphatidylethanolamine-binding protein.

Physical form

Formulated in Tris-Buffered Saline (TBS), pH 7.4, containing 1 mM Fe(NH₄)₂(SO₄)₂.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable