SRP0288

ATP Citrate Lyase Active human

recombinant, expressed in baculovirus, ≥90% (SDS-PAGE)

• Synonym(e): ACLY, ATP-citrate synthase, ATPCL, CLATP
• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ≥1.2 pmol/min-μg
• Assay: ≥90% (SDS-PAGE)
• Recombinant: expressed in baculovirus

Eigenschaften

• recombinant: expressed in baculovirus
• assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• specific activity: ≥1.2 pmol/min-μg
• mol wt: 147 kDa
• NCBI accession no.: NM_001096
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: mouse ... ACLY(47)

Beschreibung

General description

Human ATP citrate lyase (ACLY) (GenBank Accession No. NM_001096), full length, with N-terminal GST tag, MW = 147 kDa, expressed in baculovirus expression system.

Application

Active human ATP citrate lyase is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human ATP citrate lyase has been used in a study to ascertain the nature of the catalytic phosphorylation that initiates the ACL reaction, and to identity the active site residues involved. Active human ATP citrate lyase has also been used in a study to analyze tumor metabolism to reveal mitochondrial glucose oxidation in genetically diverse human glioblastomas.

Biochem/physiol Actions

ATP citrate lyase is an enzyme involved in fatty acid synthesis that generates cytosolic acetyl-CoA and oxaloacetate from citrate and CoA. ATP citrate lyase is often upregulated in cancer.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Other Notes

One unit is defined as the amount of enzyme required to convert 1 pmol of ADP to ATP/min at 37°C.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable