SRP0249

PLK2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

• Synonym(e): Polo-like kinase 2, SNK, Serum-inducible kinase
• NACRES: NA.32
• UNSPSC Code: 12352200
• Specific activity: ≥3.6 pmol/min-μg
• Assay: ≥60% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in baculovirus infected insect cells
• Concentration: >0.02 mg/mL

Eigenschaften

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• assay: ≥60% (SDS-PAGE)
• form: aqueous solution
• specific activity: ≥3.6 pmol/min-μg
• mol wt: calculated mol wt 107 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_006622
• UniProt accession no.: Q9NYY3
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PLK2(10769)

Beschreibung

General description

Human Plk2 (GenBank Accession No. NM_006622), full length, with N-terminal GST tag, expressed in a baculovirus infected Sf9 cell expression system. The theoretical MW is 107 kDa.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable