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Merck

SRP0178

Sigma-Aldrich

Histone H4 full length human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(e):

HIST2H4A

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.32

Biologische Quelle

human

Rekombinant

expressed in E. coli

Assay

≥80% (SDS-PAGE)

Form

aqueous solution

Mol-Gew.

12.1 kDa

Verpackung

pkg of 1 mg

Lagerbedingungen

avoid repeated freeze/thaw cycles

Konzentration

>0.02 mg/mL

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... HIST2H4A(8370)

Allgemeine Beschreibung

Human Histone 4 (HIST2H4A) (GenBank Accession No. NM_003548), amino acids 2-104 (end) with N-terminal His-tag, MW = 12.1 kDa, expressed in an E. coli expression system.

Anwendung

Suitable substrate for histone methyltransferases.

Biochem./physiol. Wirkung

Histones H2B and H4 are found to become mobilized during an infection with HSV-1 (herpes simplex virus-1) and become available for binding to viral genome.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot protein into single use aliquots. Store remaining undiluted protein in aliquots at -70°C. Note: Protein is very sensitive to freeze/thaw cycles.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Core histones H2B and H4 are mobilized during infection with herpes simplex virus 1.
Conn KL
Journal of Virology, 85, 13234-13252 (2011)
Recognition and classification of histones using support vector machine.
Bhasin M
Journal of Computational Biology : A Journal of Computational Molecular Cell Biology, 13, 102-112 (2006)
Shrika G Harjivan et al.
Molecules (Basel, Switzerland), 26(5) (2021-04-04)
Nevirapine (NVP), a non-nucleoside reverse transcriptase inhibitor widely used in combined antiretroviral therapy and to prevent mother-to-child transmission of the human immunodeficiency virus type 1, is associated with several adverse side effects. Using 12-mesyloxy-nevirapine, a model electrophile of the reactive
The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly.
Ahmad K and Henikoff S
Molecular Cell, 9(6), 1191-1200 (2002)
A comprehensive view of the epigenetic landscape part I: DNA methylation, passive and active DNA demethylation pathways and histone variants.
Sadakierska-Chudy A
Neurotoxicity Research, 27(1), 84-97 (2015)

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