SRP0143

PRMT4 Active human

recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE)

• Synonym(e): Arginine N-methyltransferase 4, CARM1, Coactivator-associated arginine methyltransferase 1, PRMT4
• UNSPSC-Code: 12352200
• NACRES: NA.77

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected insect cells
• Assay: ≥65% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 66 kDa
• Verpackung: pkg of 20 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_199141
• UniProt-Hinterlegungsnummer: Q86X55
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... CARM1(10498)

Beschreibung

Allgemeine Beschreibung

Human PRMT4 (CARM1) (GenBank Accession No. NM_199141), amino acids 2-end, with N-terminal His-tag, MW = 66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Einheitendefinition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.