The BioTracker™ rRNA live cell probe is a styryl quinolinium dye that selectively stains ribosomal RNA (rRNA) in mammalian cells. It has been tested on HeLa and NIH 3T3 cells. This product has not been tested in Drosophila.
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BioTracker™ rRNA Live Cell Probe
Fluorescent detection of ribosomal RNA and cell nucleoli
Synonym(e):
live cell probe, nucleoli probe, nucleolus probe, rRNA dye, rRNA probe, ribosomal RNA probe
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NACRES:
NA.31
UNSPSC Code:
12171501
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Unterstützung erhaltenGeneral description
The BioTracker™rRNA live cell probe is a styryl quinolinium dye that selectively binds ribosomal RNA (rRNA) in the cytoplasm and nucleoli of mammalian cells 1. This probe is a fluorescent small molecule that demonstrates high affinity to rRNA and therefore enables imaging of the nucleolus. Due to its low cytotoxicity, it enables detection in living systems with minimal interference.1 . As imaging technologies progress, there is increased demand for reagents that can detect diverse organoids and enable observation of subcellular functions in living cells. The optimal fluorescent reagent for live cell imaging should show high specificity to target along with low cytotoxicity 2 . Because the nucleolus is a dense organoid composed of chromatin and numerous protein components, the development of fluorescent reagents for live cell nucleoli detection is even more challenging 2,3,4. BioTracker™ rRNA live cell probe is easy to use, emits light in a longer wavelength to reduce phototoxicity and facilitate multiplexing, and can enhance nucleolar imaging dynamics.
Spectral Properties:Fluorescence images obtained by λex = 570 nm and emission at 647-667 nm.
Spectral Properties:Fluorescence images obtained by λex = 570 nm and emission at 647-667 nm.
Application
Live cell imaging probefor detection of ribosomal RNA (rRNA). Can also be used in fixed cell or tissuesamples, such as with a nuclear counterstain to identify nucleoli.
Preparation Note
Store BioTracker™ rRNA Live CellProbe at 2-8 °C, desiccated and protected from light
Note: Centrifuge vial briefly to collect contents at bottom ofvial before opening.
Note: Centrifuge vial briefly to collect contents at bottom ofvial before opening.
Analysis Note
Purity: ≥ 95% confirmed by HPLC. Molar Mass: 298 Da confirmed byLC-MS.
Legal Information
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
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| form solid | form - | form - | form - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| shipped in ambient | shipped in ambient | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level - | Quality Level 100 | Quality Level 100 |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Has this been used or tested in Drosophila?
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What is the best storage condition AFTER reconstitute in DMSO, and what is it's shelf life after being in DMSO? Is it still 4C?
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Solution stability has not been officially tested. Research and Development stored the stock solution in DMSO at 2-8 °C for 1 month. Before use, it is necessary to thaw the solution because DMSO is frozen. Frozen stock at –20 °C has not been tested.
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