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SAE0046

Sigma-Aldrich

Carboxypeptidase A aus Rinderpankreas

(Type II-PMSF treated), ≥20 units/mg protein

Synonym(e):

Carboxypolypeptidase, Peptidyl-L-aminosäure-Hydrolase

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About This Item

CAS-Nummer:
11075-17-5
EC-Nummer:
3.4.17.1 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00130718
UNSPSC-Code:
12352204
NACRES:
NA.54

Biologische Quelle

bovine pancreas

Qualitätsniveau

200

Form

aqueous suspension

Qualität

(Type II-PMSF treated)

Spezifische Aktivität

≥20 units/mg protein

Mol-Gew.

~35 kDa

Aufgereinigt durch

2× crystallization

Verunreinigungen

≤0.05 BTEE units/mg protein chymotrpsin
≤10 BAEE units/mg protein trypsin

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Carboxypeptidase A (CPA) is a secreted protease is liberated after the activation of mast cells to facilitate acute anaphylaxis. Carboxypeptidase A has a long half-life in vivo, when compared to other secreted proteases.

Anwendung

Carboxypeptidase A from bovine pancreas has been used in in vitro simulated digestion.
Carboxypeptidase A from bovine pancreas has been used in a study to investigate the expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase.

Biochem./physiol. Wirkung

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.†

Einheitendefinition

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

Angaben zur Herstellung

Treated with phenylmethylsulfonyl fluoride to eliminate trypsin and chymotrypsin activity. Dialyzed and recrystallized: aqueous suspension with toluene added.

Hinweis zur Analyse

Protein determined by E1%/278

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Quantification of Multifunctional Dipeptide YA from Oyster Hydrolysate for Quality Control and Efficacy Evaluation
Xie C L, et al.
BioMed Research International, 2018, 13288-13288 (2018)
The role of zinc in carboxypeptidase.
Bert Vallee
The Journal of Biological Chemistry, 235.1, 64-69 (1960)
Mast cell tryptase and carboxypeptidase A expression in body fluid and gastrointestinal tract associated with drug-related fatal anaphylaxis
Guo X J, et al.
World Journal of Gastroenterology, 21(47), 13288-13288 (2015)
Cheng-Liang Xie et al.
BioMed research international, 2018, 8437379-8437379 (2018-10-23)
YA is an angiotensin-I-converting enzyme- (ACE-) inhibitory peptide from oyster hydrolysate with antihypertensive activity. Its antioxidant and anti-inflammatory activity were investigated in this study. YA can dose-dependently quench DPPH and ABTS radical and inhibit lipopolysaccharide-induced nitric oxide in RAW 264.7

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