This product is tested for unit of activity per mL, so the volume varies and is lot-dependent. Please refer to the sample Certificate of Analysis for enzyme activity.
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SAE0023
Alanine Racemase from Bacillus stearothermophilus
buffered aqueous solution, ≥10 IU/mg solid
Synonym(e):
Alanine Racemase from Bacillus stearothermophilus
zur Ansicht der Organisations- und Vertragspreise.
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 units | Warenkorb auf Verfügbarkeit prüfen | € 528,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥10 IU/mg solid
Biological source:
Bacillus sp. (B. stearothermophilus)
€ 528,00
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Technischer Dienst
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Unterstützung erhaltenbiological source
Bacillus sp. (B. stearothermophilus)
Quality Segment
form
buffered aqueous solution
specific activity
≥10 IU/mg solid
mol wt
Mr 78 kDa (2 subunits 39 kDa each)
storage temp.
−20°C
Application
Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-14C]alanine to a racemic mixture of L/D- [14C]alanine †.
Biochem/physiol Actions
Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†
Physical form
Aqueous solution containing 10 mM potassium phosphate
Other Notes
One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| specific activity ≥10 IU/mg solid | specific activity ~30 units/mg protein (Lowry) | specific activity ≥0.75 Anson units/mL | specific activity - |
| biological source Bacillus sp. (B. stearothermophilus) | biological source Bacillus subtilis | biological source Bacillus licheniformis | biological source Bacillus sp. (Bacillus cereus) |
| form buffered aqueous solution | form buffered aqueous glycerol solution | form liquid | form lyophilized |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| mol wt Mr 78 kDa (2 subunits 39 kDa each) | mol wt - | mol wt - | mol wt - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the concentration and/or volume of this solution?
1 answer-
Helpful?
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What is the amino acid sequence of the Bacillus stearothermophilus alanine racemase that you are selling (SAE0023)?
1 answer-
SAE0023 is isolated from Bacillus sp. The published sequence can be found here:
https://www.uniprot.org/uniprotkb/P10724/entryHelpful?
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