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Merck

SAB5700599

Sigma-Aldrich

Recombinant 2019-nCoV Spike RBD Protein with mFc tag

Synonym(e):

2019-nCoV RBD Protein, NCP-CoV RBD Protein, S glycoprotein Subunit1 RBD Protein, S1-RBD protein, novel coronavirus RBD Protein

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.26

Rekombinant

expressed in HEK 293 cells

Qualitätsniveau

Assay

>95% (SDS-PAGE)

Form

lyophilized powder

Mol-Gew.

50.8 kDa

Verunreinigungen

<0.1 EU/μg protein (LAL test)

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

SARS virus ... S(43740568)

Allgemeine Beschreibung

Recombinant SARS-COV-2(2019-nCoV) Spike RBD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of SARS-COV-2(2019-nCoV) Spike RBD (Accession #YP_009724390.1) fused with a mFc tag at the C-terminus.

Biochem./physiol. Wirkung

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 Protein at 2μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 Spike RBD-mFc Protein,the EC50 of Recombinant SARS-COV-2 Spike RBD-mFc Protein is 2.58 ng/mL.

Rekonstituierung

For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Lagerung und Haltbarkeit

Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8 °C for up to 1 week. This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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