SAB4200150

Anti-PIWIL3 (N-terminal) antibody produced in rabbit

~1.0 mg/mL

• Synonym(e): Anti-HIWI3, Anti-Piwi-like protein 3
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: ~101 kDa
• Speziesreaktivität: human
• Konzentration: ~1.0 mg/mL
• Methode(n): immunohistochemistry: suitable using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections and biotin/ExtrAvidin^®-Peroxidase staining system; western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human PIWIL3
• UniProt-Hinterlegungsnummer: Q7Z3Z3
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

The PIWIL3 (Piwi like RNA-mediated gene silencing 3) protein belongs to the Piwi subfamily of Argonaute proteins. The Argonaute proteins contain a Piwi Argonaute-Zwille (PAZ) domain and PIWI domain.[1][2] In humans, there are four PIWI proteins. The PIWIL3 gene is mapped to human chromosome 22q11.23.[3] The Piwi proteins are mostly localized to the germline, where they bind to PIWI-interacting RNAs (piRNAs).[1]

Spezifität

Anti-PIWIL3 (N-terminal) recognizes human PIWIL3.

Anwendung

Anti-PIWIL3 (N-terminal) antibody is also suitable for immunohistochemistry.

Anti-PIWIL3 (N-terminal) antibody produced in rabbit has been used in western blotting.[4]

Biochem./physiol. Wirkung

The P-element Induced WImpy testis (Piwi) proteins are important for the biogenesis and function of small non-coding RNAs. These proteins and PIWI-interacting RNAs (piRNAs) have been implicated in epigenetic control of gene expression, transposon silencing, gene expression, and translation regulation. The Piwi proteins significantly participate in germline development and gametogenesis of various metazoan species.[2] These proteins undergo symmetrical dimethyl arginines (sDMAs) modification by protein methyltransferase 5 (PRMT5).[5] The Piwi proteins are implicated in various cancers such as colorectal, breast, bladder, ovarian and non-small lung cancer and many more. Increased PIWIL3 gene expression has been observed in gastric cancer and metastatic melanoma.[3]

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Lagerung und Haltbarkeit

Store at −20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilutions should be discarded if not used within 12 hours.

Rechtliche Hinweise

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable