S7199

Monoclonal Anti-SERCA3 antibody produced in mouse

~1.0 mg/mL, clone PL/IM430, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Anti-ATP2A3, Anti-ATPase, Ca(2+)-Transporting, Ubiquitous, Anti-Sarcoplasmic reticulum Ca(2+)-ATPase 3
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: PL/IM430, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~100 kDa
• Speziesreaktivität: human
• Konzentration: ~1.0 mg/mL
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.25-0.5 μg/mL using platelet total cell extract.
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: Q93084
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ATP2A3(489)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-SERCA3 (mouse IgG1 isotype) is derived from the hybridoma PL/IM430 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The ATPase sarcoplasmatic/endoplasmatic reticulum Ca²⁺ transporting 3 (ATP2A3) gene codes for Ca²⁺-ATPase3 from the sarco/endoplasmic reticulum (SERCA3) and the coded protein is expressed in endothelial and epithelial tissues. The human SERCA3 gene gives rise to six 3¢-end splice variants encoding proteins varying in their C-terminal part and termed SERCA3a-f. SERCA3 gene is mapped to human chromosome 17p13.3.

Spezifität

Monoclonal Anti-SERCA3 recognizes human SERCA3.

Anwendung

Monoclonal Anti-SERCA3 antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry
• western blotting
• immunoprecipitation
• immunohistochemistry

Biochem./physiol. Wirkung

Sarco/endoplasmic calcium ATPase pumps (SERCA) protein plays a pivotal role in intracellular calcium [Ca²⁺] homeostasis by lowering the cytoplasmic [Ca²⁺] concentration. Expression of sarco/endoplasmic reticulum (SERCA3) gene is reduced in gastric/colon carcinoma and leukemia cell types.The ATPase sarcoplasmatic/endoplasmatic reticulum Ca²⁺ transporting 3 (ATP2A3) gene may serve as an important target for salinomycin. Overexpression of this gene stimulates apoptosis. SERCA3 protein plays an important role in Ca²⁺ transport.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)