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S1452

MISSION® siRNA Transfection Reagent

Synonym(e):

MISSION® siRNA Transfection Reagent

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Über diesen Artikel

NACRES:
NA.51
UNSPSC Code:
41106502


product line

MISSION®

Quality Segment

form

aqueous solution

shipped in

wet ice

storage temp.

2-8°C

General description

The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.

Other Notes

Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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Dieser Artikel
71115SHC202VSHC002V
description

-

description

Efficient delivery of siRNA for targeted gene suppression

description

Targets no known mammalian genes

description

Targets no known mammalian genes

product line

MISSION®

product line

-

product line

MISSION®

product line

MISSION®

form

aqueous solution

form

liquid

form

-

form

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−70°C

storage temp.

−70°C

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice


pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Lagerklasse

3 - Flammable liquids

wgk

WGK 3

flash_point_f

120.2 °F

flash_point_c

49 °C



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