RAB0019

Human Amphiregulin ELISA Kit

for serum, plasma, cell culture supernatant and urine

• Synonym(e): AR
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... AREG(374)
• Input: sample type plasma; sample type cell culture supernatant(s); sample type urine; sample type serum
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Eigenschaften

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type plasma; sample type cell culture supernatant(s); sample type urine; sample type serum
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 10 pg/mL; standard curve range: 16.46-4000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... AREG(374)

Beschreibung

General description

The Human Amphiregulin (AR) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human AR in serum, plasma, cell culture supernatants and urine.
The AREG (amphiregulin) gene codes for a shedding ectodomain, derived from a transmembrane precursor of 252 amino acids. The encoded protein is released into blood or cellular vicinity through autocrine and paracrine.[1][2] Areg is predominantly expressed in epithelial, mesenchymal cells and also by active immune cells.[1] The AREG gene is mapped to human chromosome 4q13.3.[2]

Immunogen

Recombinant Human Amphiregulin

Application

Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Amphiregulin ELISA Kit has been used to estimate the plasma concentration of amphiregulin by ELISA.[3]

Biochem/physiol Actions

Areg (amphiregulin) stimulates growth-signaling pathways via EGF (epidermal growth factor) receptors. Areg participates in the organogenesis of mammary gland. It is regarded as a pro-oncogenic factor and contributes to tumor progression. Upregulation of the AREG gene is observed in cancer and resists chemotherapy.[1] AREG reduces E-cadherin expression in order to promote invasion and progression of ovarian cancer.[4]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Sicherheitshinweise

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Lagerklasse: 10 - Combustible liquids