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Merck

R7876

Sigma-Aldrich

Ribonukleinsäure, Transfer aus Weizenkeimen

Type V, 15-19 units/mg solid

Synonym(e):

Transfer-RNA, tRNA

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About This Item

CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352106
NACRES:
NA.55

Qualität

for molecular biology

Qualitätsniveau

Typ

Type V

Form

lyophilized powder

Spezifische Aktivität

15-19 units/mg solid

Lagerbedingungen

desiccated

Löslichkeit

H2O: ~2 mg/mL, clear to hazy

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Wheat germ tRNA contains many different specific tRNA molecules.

Anwendung

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.

Komponenten

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Einheitendefinition

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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