P7778

Phospholipase A₂ aus Naja mossambica mossambica

lyophilized powder, ~1,500 units/mg protein

• Synonym(e): PLA₂
• CAS-Nummer: 9001-84-7
• UNSPSC Code: 12352204
• EC Number: 232-637-7
• MDL number: MFCD00131890
• EG-Nummer: 3.1.1.4 (BRENDA, IUBMB)

Eigenschaften

• form: lyophilized powder
• specific activity: ~1,500 units/mg protein
• composition: Protein, ~90%
• pI: 8.8 (isoenzyme)
• storage temp.: −20°C

Beschreibung

Biochem/physiol Actions

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.9 at 25 °C.

The toxic phospholipase A₂ from Naja mossambica mossambica.

Sicherheitshinweise

• Lagerklasse: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)