Percoll and Percoll Plus are manufactured by Cytiva. The Product Information Sheet for this product provides a basic protocol and references for common cell types. Additional references and mathematical models can be found on the Cytiva website.
Typically, references identify a specific density for each cell type or molecule of interest. Instructions for preparing a specific density are included in the product information sheet or a similar document on the Cytiva website. The references will mention the G force to be used, as it accurately defines the centrifuge conditions. If revolutions per minute (RPM) is stated, the radius of the centrifuge affects the outcome. Nomogram tables are available for converting G force to RPM. To find a nomogram table, search for "nomogram" in the document titled "Centrifugal Filters: Guide to Converting g-Force to RPM." Instructions for use are included to convert G force to the appropriate RPM setting for a specific centrifuge.
Fortfahren mit
P4937
Percoll®
pH 8.5-9.5 (25 °C), suitable for cell culture
Synonym(e):
Dichtegradientenmedium
Größe auswählen
| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
25 mL | Warenkorb auf Verfügbarkeit prüfen | € 128,00 |
100 mL | Warenkorb auf Verfügbarkeit prüfen | € 313,00 |
500 mL | Warenkorb auf Verfügbarkeit prüfen | € 1.110,00 |
Über diesen Artikel
sterility
aseptically filled
Quality Segment
form
colloidal
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
pH
8.5-9.5 (25 °C)
density
1.13 g/mL±0.005 g/mL at 25 °C (lit.)
storage temp.
2-8°C
General description
Application
Features and Benefits
- Die geringe Osmolalität ermöglicht eine genaue Anpassung an physiologische Bedingungen ohne signifikante Störung durch das Medium.
- Kompatibel mit lebenden Zellen und Viren, was eine Trennung und Rückgewinnung von intakten, vollständig aktiven Systemen ermöglicht.
- Undurchlässig für biologische Membranen, was zu keiner Veränderung der Schwebedichte von Partikeln beim Zentrifugieren führt.
- Die spontane Bildung eines Gradienten beim Zentrifugieren ermöglicht das Mischen großer Probenvolumina in den Zentrifugenröhrchen.
- Die geringe Viskosität führt zur schnellen Bildung von Gradienten und zur Partikelabscheidung.
- Die meisten biologischen Partikel mit Sedimentationskoeffizentenwerten von mehr als 60S können erfolgreich in Percoll-Gradienten isoliert werden.
Legal Information
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| sterility aseptically filled | sterility aseptically filled | sterility aseptically filled | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | plant: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable |
| density 1.13 g/mL±0.005 g/mL at 25 °C (lit.) | density - | density - | density - |
| form colloidal | form colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) ) | form colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) | form liquid |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 500 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Lagerklasse
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Further to the above question: "When using Product P1644, Percoll®, what density should I use for my particular system?", when I review the sheet for granulocytes and RBCs it gives a centrifugation speed as "*" . What does this mean?
1 answer-
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Is there a "best" salt solution to use with Product P1644, Percoll®?
1 answer-
Percoll can be used with various salt formulations (PBS, 1.5 M NaCl, HBSS, etc.). Physiological saline and balanced salt solutions can be suggested. Sucrose is included to prevent aggregation. You can find detailed preparation instructions on the product information sheet (under Documents, above) for this product.
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Can Percoll®, Product P1644, be autoclaved?
1 answer-
Percoll can be autoclaved, but this must be done without any salts or sucrose present.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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When using Product P1644, Percoll®, what density should I use for my particular system?
1 answer-
The product information sheet (under Documents, above) has a table of densities for different organelles and cell types.
Helpful?
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Can you help me calculate gradients for Percoll®, Product P1644?
1 answer-
The product information sheet (under Documents, above) has an equation for calculating density of a given solution.
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What is the difference between Percoll® Products P4937 and P1644?
1 answer-
Product Nos. P1644 and P4937 are both Percoll. Product No. P4937 has additional testing for use in cell culture.
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