Percoll is produced by Cytiva, formerly known as GE Life Sciences. Details regarding the proprietary manufacturing process are not publicly available. However, it can be mentioned that the Percoll particles range in size from 15 to 30 nanometers. While the density of Percoll solution is measured, the specific information regarding the amount of coating on the silica particles is not provided.
Fortfahren mit
P1644
Percoll®
pH 8.5-9.5 (20 °C)
Synonym(e):
Percoll-Medien für Dichtegradienten
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
25 mL | Warenkorb auf Verfügbarkeit prüfen | € 71,50 |
100 mL | Warenkorb auf Verfügbarkeit prüfen | € 203,00 |
500 mL | Warenkorb auf Verfügbarkeit prüfen | € 620,00 |
1 L | Warenkorb auf Verfügbarkeit prüfen | € 1.090,00 |
Über diesen Artikel
Technischer Dienst
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Unterstützung erhaltensterility
aseptically filled
Quality Segment
form
colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) )
technique(s)
cell culture | mammalian: suitable
color
clear colorless to faint yellow
pH
8.5-9.5 (20 °C)
application(s)
hematology
histology
storage temp.
2-8°C
General description
Percoll® ist ein klassisches Medium für die Dichtegradientenzentrifugation von Zellen, Viren und subzellulären Partikeln. Percoll besteht aus kolloidalen Kieselgelpartikeln mit einem Durchmesser von 15-30 nm (23 % w/w in Wasser), die mit Polyvinylpyrrolidon (PVP) beschichtet sind. Aufgrund der PVP-Beschichtung ist das Medium vollkommen ungiftig und ideal zur Verwendung mit biologischen Materialien geeignet.
Application
Percoll® wurde bei der Spermienpräparation zur Untersuchung des Energiebedarfs bovinen Embryonen eingesetzt.
Percoll besteht aus kolloidalen Kieselgelpartikeln mit einem Durchmesser von 15 - 30 nm (23 % w/w in Wasser), die mit Polyvinylpyrrolidon (PVP) beschichtet sind. Es wird bei der Zellseparation und bei der Isolierung von Organellen eingesetzt.
Percoll besteht aus kolloidalen Kieselgelpartikeln mit einem Durchmesser von 15 - 30 nm (23 % w/w in Wasser), die mit Polyvinylpyrrolidon (PVP) beschichtet sind. Es wird zur Herstellung nicht-toxischer, niedrigviskoser, niedrigosmolarer Dichtegradienten verwendet, die zur Isolierung von Zellen, Organellen und Viren eingesetzt werden können. Es wurde zur Trennung von Vesikeln der inneren und äußeren Membran von E. coli verwendet. Percoll ist isoosmotisch über den gesamten Gradienten, nicht toxisch und einfach aus dem aufgereinigten Material zu entfernen.
Legal Information
Percoll is a registered trademark of Cytiva
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Dieser Artikel | |||
|---|---|---|---|
| sterility aseptically filled | sterility aseptically filled | sterility aseptically filled | sterility aseptically filled |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | plant: suitable | technique(s) ELISA: suitable, electrophoresis: suitable, western blot: suitable |
| form colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) ) | form colloidal | form colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) | form solution |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| pH 8.5-9.5 (20 °C) | pH 8.5-9.5 (25 °C) | pH 8.9 (20 °C) | pH - |
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Lagerklasse
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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What is the details about the manufacturing process of Percoll, specifically regarding the amount of PVP used during bead soaking or the approximate PVP concentration within Percoll?
1 answer-
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What is procedure for sperm preparation for ivf with percoll gradient
1 answer-
Please note that this product is intended for research use only. This material is not tested for the isolation of sperm samples. The following reference may be helpful:
Chen. Y. et al., Int. J. Fertil., 37, 315-319 (1992).Please see the link below to review the product datasheet for additional information.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/977/430/p1644pis.pdfHelpful?
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CAN I FREEZE PERCOLL (P1644)? I SEE IN OTHER REF THAT I CAN, BUT NOT IN THIS REF
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This material should not be stored frozen for long periods of time. As per the product information sheet, if stored at –20 °C, gradients form upon thawing, necessitating mixing the contents of the bottle before use. Please see the link below to review the product datasheet for further information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/426/291/percoll-product-info-sheet-ms.pdfHelpful?
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Percoll (P1644) 을 gradient 만드는데 사용했는데요, P4937도 같은 용도로 사용 가능한가요?
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Yes, product P4937 can be used for the same use as product P1644.
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I often find crystals. Can I filter the solution before use? What filter size could I use without affecting the functionality of the product?
1 answer-
Particles may be removed by filtration or low speed centrifugation. More information about the particles filtration can be found in the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/977/430/p1644pis.pdf
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/426/291/percoll-product-info-sheet-ms.pdfHelpful?
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When using Product P1644, Percoll®, what density should I use for my particular system?
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The product information sheet (under Documents, above) has a table of densities for different organelles and cell types.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the difference between Percoll® Products P4937 and P1644?
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Product Nos. P1644 and P4937 are both Percoll. Product No. P4937 has additional testing for use in cell culture.
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Can you help me calculate gradients for Percoll®, Product P1644?
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The product information sheet (under Documents, above) has an equation for calculating density of a given solution.
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Is there a "best" salt solution to use with Product P1644, Percoll®?
1 answer-
Percoll can be used with various salt formulations (PBS, 1.5 M NaCl, HBSS, etc.). Physiological saline and balanced salt solutions can be suggested. Sucrose is included to prevent aggregation. You can find detailed preparation instructions on the product information sheet (under Documents, above) for this product.
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