O1139

Anti-Osteoprotegerin antibody produced in goat

affinity isolated antibody, lyophilized powder

• Synonym(e): Anti-OPG
• UNSPSC Code: 51111800
• NACRES: NA.41
• MDL number: MFCD03702437
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, Neutral, WB
• Citations: 8

Eigenschaften

• biological source: goat
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: mouse
• technique(s): immunohistochemistry: 5-15 μg/mL, neutralization: suitable, western blot: 0.1-0.2 μg/mL
• UniProt accession no.: O08712
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Tnfrsf11b(18383)

Beschreibung

General description

Osteoprotegerin (OPG) secreted by osteoblasts and osteogenis stromal stem cells belongs to the TNF receptor superfamily. OPG is expressed in bone marrow, osteoblasts, heart, lung, kidney, thymus, B lymphocytes, chondrocytes and smooth muscle cells. The ratio of OPG and receptor activator of NF-κB Ligand (RANKL) is crucial determining the bone mass. Receptor activator of NF-κB (RANK)/RANKL/OPG axis decides the bone biology. OPG binds RANKL and prevents the activation of osteoclasts, the cells that deplete bone mass. Excess levels of OPG lead to osteopetrosis while deficiency results in osteoporosis. The RANK/RANKL/OPG pathway mediates the activation of multiple pathways such as NF-κB, Akt, JNK, and MAPK
Anti-osteoprotegerin specifically reacts with recombinant mouse OPG. The antibody may cross react with human OPG (25% homology).

Immunogen

purified recombinant mouse osteoprotegerin expressed in mouse NSO cells.

Application

Anti-osteoprotegerin antibody may be used to neutralize mouse OPG at neutralization dose (ND50) of 0.5-2 μg/ml. The antibody is suitable for immunoblotting at a working concentration of 0.1-0.2 μg/ml.

Biochem/physiol Actions

The antibody has the ability to neutralize the biological activity of recombinant mouse OPG.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)