NA0600

GenElute^™ HP Endotoxin-Free Plasmid Megaprep Kit

1 kit sufficient for 5 preparations

• Synonym(e): Gen Elute, GenElute Endotoxin-Free Plasmid Kit
• NACRES: NA.52
• UNSPSC Code: 41105501

Eigenschaften

• Quality Segment: 200
• usage: kit sufficient for 5 preparations
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• test parameters: sample volume: 200-1000 mL bacterial culture
• greener alternative category: , Aligned
• storage temp.: 15-25°C

Beschreibung

General description

The GenElute HP Endotoxin-Free Plasmid Megaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 5 mg of high copy plasmid DNA with ≤ 0.1 endotoxin units/μg can be recovered from 200 mL - 1.0 L LB medium or 200 - 600 mL TB medium of E. coli culture in less than 1.5 hours. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digest, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Click here to view the kit protocol

Application

GenElute^™ HP Endotoxin-Free Plasmid Megaprep Kit has been used to purify plasmid DNA.

High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

Features and Benefits

• Up to 5 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
• Only 90 minutes from pelleted cells to purified plasmid
• Vacuum format with no ethanol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit-Komponenten auch einzeln erhältlich

• G3546

  GenElute^™ HP Pro Megaprep Binding Columns 5

  SDB
• C2112

  Column Preparation Solution 2

  SDB
• N7411

  Neutralization Solution EF

  SDB
• L1912

  Lysis Solution

  SDB
• 2107

  E-Toxate^™ Water, endotoxin, free

  SDB
• W4639

  Wash Solution 2

  SDB
• R1149

  Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )

  SDB

Alle anzeigen (11)

Sicherheitshinweise

• pictograms: GHS02,GHS08,GHS05,GHS07
• signalword: Danger
• hcodes: H226,H290,H302 + H332,H315,H319,H334,H336,H372
• pcodes: P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
• target_organs: Central nervous system, Lungs
• Lagerklasse: 3 - Flammable liquids
• flash_point_f: 77.0 °F - closed cup
• flash_point_c: 25 °C - closed cup
• wgk: WGK 3