N4773

Neuron-specific enolase from human brain

≥95% (SDS-PAGE), buffered aqueous solution

• Synonym(e): 2-Phospho-D-glycerate hydro-lyase, Enolase, Neuron-specific from human brain, NSE, Thermolabile antigen A
• EG-Nummer: 4.2.1.11 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00081785
• Specific activity: ≥10 units/mg protein
• Assay: ≥95% (SDS-PAGE)
• Biological source: human brain

Eigenschaften

• biological source: human brain
• Quality Segment: 200
• assay: ≥95% (SDS-PAGE)
• form: buffered aqueous solution
• specific activity: ≥10 units/mg protein
• UniProt accession no.: P09104
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... ENO2(2026)

Beschreibung

General description

Neuron-specific enolase (NSE) is a neuronal form of the glycolytic enzyme enolase, which was first found in extracts of brain tissue.

Application

Neuron-specific enolase from human brain has been used in a study to assess human amniotic mesenchymal stem cells in the treatment of focal cerebral ischemia. It has also been used in a study to investigate sinonasal teratocarcinosarcoma with rhabdoid features.

Biochem/physiol Actions

Neuron-specific enolase promotes survival of neurons and can provide neuroprotective effects by binding to neurons in a calcium-dependent manner.

Neuron-specific enolase (NSE) is expressed in all neuronal cell types, and its expression marks the acquisition of synaptic function. Following acute neuronal injury, NSE levels are increased in neuronal cell bodies. Increased levels of NSE in serum and cerebrospinal fluid have been used as markers for injury and neuronal cell death. Tumors derived from many cell types, including most neuronal and neuroendocrine tumors, express NSE.

Physical form

Solution in 100 mM Tris-HCl, 5 mM MgSO₄, 250 mM KCl, pH 5.0-5.2

Other Notes

One unit causes the formation of 1.0 μmole of phospho(enol)pyruvate per minute at pH 6.8 at 25 °C

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable