N2786

Monoclonal Anti-Nedd8 antibody produced in mouse

clone ND23, purified immunoglobulin, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01865547
• Conjugate: unconjugated
• Clone: ND23, monoclonal
• Application: ARR, WB
• Citations: 4

Eigenschaften

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: ND23, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): microarray: suitable, western blot: 0.15-0.3 μg/mL using recombinant GST-Nedd8
• isotype: IgG2b
• UniProt accession no.: Q15843
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NEDD8(4738)

Beschreibung

General description

Monoclonal Anti-Nedd8 (mouse IgG2b isotype) is derived from the ND23 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human Nedd8 conjugated keyhole limpet hemocyanin (KLH). Nedd8 is a small protein (9 kDa) that shows the highest similarity to the ubiquitin protein (60% amino acid sequence identity).

Neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) is an ubiquitin-like, 76 amino acid protein. It is expressed in endocrine and epithelial cells. NEDD8 belongs to the ubiquitin-like protein superfamily.

Immunogen

synthetic peptide corresponding to amino acids 20-32 of human Nedd8, conjugated to KLH.

Application

Monoclonal Anti-Nedd8 antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

Biochem/physiol Actions

Neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) is involved in pathways concerned with protein degradation. It targets substrates for neddylation, which is a post-translational modification. NEDD8 also regulates the signaling of proteins like cullins.

Nedd8 conjugation has a physiological role in processes where the stability of nuclear factor kappa light chain enhancer of activated B cells (NF-κB) and p27 is important.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2