This product is suitable for the detection of Nitric Oxide in animal tissues (liver, kidney, etc.), cell culture (adherent or suspension cells), serum, plasma, and urine. Suitability for food samples has not been tested.
MAK367
Nitrite Assay Kit (Griess Reagent)
sufficient for 200 colorimetric tests
Synonym(e):
Nitrite Detection Kit
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NACRES:
NA.84
UNSPSC Code:
12161503
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Unterstützung erhaltenusage
sufficient for 200 colorimetric tests
detection method
colorimetric
relevant disease(s)
immunological diseases
storage temp.
2-8°C
General description
Nitrogen-based ions, nitrite and nitrate, are found in almost every living organism. Furthermore, endogenous Nitrite levels are found in mammals and can also be obtained from dietary sources. In humans, nitrite is further metabolized to Nitric Oxide and other reactive nitrogen species (nitrogen oxides). The Nitrate-Nitrite-NO biochemical pathway is well known for its participation in cell signaling, hypoxiadependent response and regulation of blood flow. Recent studies suggest the reduction of Nitrite to Nitrogen Oxygen in the mitochondria. Specifically, myoglobin and xanthine oxidoreductase could generate NO under hypoxic conditions leading to mitochondrial respiration.
Application
- Convenient environmentally friendly on-site quantitative analysis of nitrite and nitrate in seawater based on polymeric test kits and smartphone application.: This research develops an eco-friendly and portable method for nitrite and nitrate analysis in seawater, employing polymeric test kits and a smartphone application for data analysis, facilitated by the Griess reagent for nitrite detection. (Choodum et al., 2020).
- MiR-124 inhibits spinal neuronal apoptosis through binding to GCH1.: The study investigates the role of miR-124 in preventing spinal neuronal apoptosis, utilizing a nitrite assay kit to measure nitric oxide levels as part of the biochemical analysis. (Yuan et al., 2019).
- Humoral factors in ALS patients during disease progression.: This paper examines the changes in humoral factors in ALS patients, measuring nitric oxide levels using a nitrite assay kit to assess inflammation and oxidative stress. (Ehrhart et al., 2015).
- Inducible nitric oxide synthase-nitric oxide plays an important role in acute and severe hypoxic injury to pancreatic beta cells.: The research highlights the significance of nitric oxide in hypoxic injury to pancreatic beta cells, using a nitrite assay kit to quantify nitric oxide levels. (Ko et al., 2008).
The kit is suitable for the detection of Nitric Oxide in animal tissues (liver, kidney, etc.), cell culture (adherent or suspension cells), serum, plasma and urine.
Biochem/physiol Actions
The Nitrite Assay Kit utilizes the Griess Reagent, a classic protocol for the estimation of nitrite. In the assay, nitrite is reduced to Nitrogen Oxide using Griess Reagent I. Then, Nitrogen Oxide reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at A540 nm. The one-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.
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Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
Lagerklasse
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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Eloi Franco-Trepat et al.
British journal of pharmacology, 179(2), 270-286 (2021-10-14)
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The British journal of nutrition, 1-14 (2022-01-11)
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Verwandter Inhalt
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Can this kit be used for food samples?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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In the protocol, it is says, that I should seed my cells at 1x10 ^(6). Will I still have good result if I seed my cells 1x10^(4) instead?
1 answer-
This kit was designed to measure nitrite levels in biological samples. For cultured mammalian cells, it is recommended to use one million (1x10 ^(6)) cells per sample. If you use only ten thousand (1x10^(4)) cells per sample, the level of nitrite from that amount of cells may be below the limits of detection for this kit. Please note that this information refers to the number of cells per sample; it does not refer to how many cells may have been originally seeded on the plate.
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Can this kit be used to examine nitric oxide release from polymer fibers?
1 answer-
The kit is suitable for samples such as animal tissues (liver, kidney, etc.), cell culture (adherent or suspension cells), serum, plasma, and urine. No specific experiments have been performed for the analysis in a polymer fiber sample.
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