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Merck

MAK341

Sigma-Aldrich

Deproteinizing Sample Preparation Kit

sufficient for 200 assays

Synonym(e):

Deproteinization Kit

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 200 assays

Anwendung(en)

sample preservation

Allgemeine Beschreibung

The presence of protein and various enzyme activities frequently interferes with the analysis of small molecules in biological samples. Many bioassays require removal of proteins from samples prior to analysis. Among the deproteinization protocols developed over the last half century, perchloric acid (PCA) precipitation has been extensively used in many different sample preparation procedures, since not only does it remove most of the protein present but it also functions to stabilize many of the small molecule analytes. PCA deproteinization methods have been successfully used in the preparation samples prior to quantitation of an array of small molecules, including glycogen, ATP, cAMP, glutathione, antioxidants, etc.

Anwendung


  • Protein Removal for LC-MS/MS in Clinical Biochemistry: The deproteinizing sample preparation kit facilitated the removal of proteins from whole blood using paramagnetic micro-particles, enhancing the accuracy of LC-MS/MS analyses in clinical settings (Van den Bossche et al., 2014).

  • Efficient Sample Prep for Metabolomics Research: The kit was applied to deproteinize serum samples using magnetic micro-particles, proving essential for reliable LC-MS/MS analysis in clinical biochemistry, particularly for metabolomics research (König et al., 2013).

Eignung

Suitable for deproteinizing various biological samples

Prinzip

The Deproteinizing Sample Preparation Kit utilizes a PCA precipitation method, providing a unique tool to prepare samples for the analysis of a variety of small molecules

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Ox. Liq. 2 - Skin Corr. 1A - STOT RE 2

Zielorgane

Thyroid

Lagerklassenschlüssel

5.1B - Oxidizing hazardous materials

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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