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MAK263

Kolorimetrisches/Fluorometrisches Glucose-Assay-Kit

sufficient for 100 colorimetric or fluorometric tests

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NACRES:
NA.84
UNSPSC Code:
12161503

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usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric, fluorometric

relevant disease(s)

endocrinological disorders, diabetes

storage temp.

−20°C

General description

Glucose ist eine primäre Energiequelle, die natürlicherweise in ihrem freien Zustand in Früchten und anderen Pflanzenteilen vorkommt. Anormale Glucosespiegel werden mit verschiedenen Stoffwechselstörungen wie Hypoglykämie, Hyperglykämie und Diabetes mellitus in Verbindung gebracht. Zur Diagnose von glucosebedingten Erkrankungen werden häufig Messungen des Glucosespiegels in Geweben und Körperflüssigkeiten (wie Blut und Urin) herangezogen. Der Glucosespiegel wird auch überwacht, um die Wirksamkeit von Therapeutika wie Insulin und Sulfonylharnstoffen bei Typ-2-Diabetikern zu überprüfen.

Application

Geeignet für die Bestimmung von Glucosekonzentrationen in verschiedenen biologischen Proben, einschließlich Serum, Plasma, Lebensmittel oder Wachstumsmedium. Dieses Kit eignet sich ebenfalls zur Überwachung des Glucosegehalts während der Fermentation und der Glucosezufuhr in Proteinexpressionsprozessen.

Biochem/physiol Actions

Glucose wird oxidiert, um ein kolorimetrisches (570 nm)/fluorometrisches (λex = 535/λem = 587 nm) Produkt zu erzeugen, das proportional zur Menge der vorhandenen Glucose ist. Mit dem Kit können 1–10.000 μM Glucose in unterschiedlichen Proben nachgewiesen werden.

Features and Benefits

Kompatibel mit Handhabungssystemen mit hohem Durchsatz.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334,H412

Hazard Classifications

Aquatic Chronic 3 - Resp. Sens. 1

Lagerklasse

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
8J20K06060
8H05K06060
5K30K06060
J20K06060
8F22K06060

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

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Urinary tract infections (UTIs) are mainly caused by uropathogenic Escherichia coli (UPEC). Acute and recurrent UTIs are commonly treated with antibiotics, the efficacy of which is limited by the emergence of antibiotic resistant strains. The natural sugar d-mannose is considered
Gemma K Kinsella et al.
International journal of molecular sciences, 22(19) (2021-10-14)
GPR21 is a constitutively active, orphan, G-protein-coupled receptor, with in vivo studies suggesting its involvement in the modulation of insulin sensitivity. However, its precise contribution is not fully understood. As the liver is both a major target of insulin signalling
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Hypoxia is an important environmental factor and has been correlated with tumor progression, treatment resistance and poor prognosis in many solid tumors, including triple-negative breast cancer (TNBC). Emerging evidence suggests that long noncoding RNA (lncRNA) functions as a critical regulator
Ryan K Perkins et al.
Nutrients, 11(3) (2019-03-03)
The purpose of this investigation was to evaluate the effects of experimental hyperglycemia on oxidative damage (OX), advanced glycation end products (AGEs), and the receptor for AGEs (RAGE) through an in vivo approach. Obese subjects (n = 10; 31.2 ±
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Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Can this kit be used on solid tissue samples? If so, how should the tissue be homogenized?

    1 answer

    1. This kit can be used for tissue samples. To homogenize, please proceed as follows:
      Homogenize the tissue sample in assay buffer using the following protocol:

      Start with approximately 10 mg of tissue (the amount may need to be optimized), wash in cold PBS and resuspend in ~100 uL of assay buffer provided with the kit.
      Homogenize using a Dounce homogenizer or a pestle, on ice to keep the sample cold (10-15 passes).
      Centrifuge at 4°C in a cold microcentrifuge to remove insoluble.
      Collect the supernatant and keep it on ice throughout sample preparation.

      Use a few different volumes of this supernatant (between 2 and 50 uL) and bring them up to a total volume of 50 µl/well with Glucose Assay Buffer in a 96-well plate, as recommended for a typical liquid sample.
      The reason for trying different volumes of samples is to ensure the readings are within the standard curve range.
      Then proceed with the 'Assay Reaction' step.

      Please see the datasheet below for the assay reaction step:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/275/602/mak263bul.pdf

      Helpful?

  4. We require a protocol for #MAK263 for my customer

    1 answer

    1. Please see the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/275/602/mak263bul.pdf

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