MAK162

Colorimetric Maleimide Assay Kit

sufficient for 100 colorimetric tests

• NACRES: NA.84
• UNSPSC Code: 12161503

Eigenschaften

• usage: sufficient for 100 colorimetric tests
• detection method: colorimetric
• relevant disease(s): cancer
• storage temp.: −20°C

Beschreibung

General description

Maleimide is an unsaturated imide used in protein conjugation reactions. The direct measurement of maleimide moieties following conjugation reactions by spectrophotometric analysis at 302 nm is insensitive due to the small extinction coefficient (620M-1 cm-1) and complicated by protein absorbance at this wavelength.

Application

Suitable for quantifying maleimide groups

Biochem/physiol Actions

The colorimetric maleimide quantiation kit provides a simple and direct procedure for measuring maleimide groups. The sample is first reacted with a known amount of excess thiol and then the remaining unreacted thiol is assayed using 4,4′-DTDP (4,4′-Dithiodipyridine). The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H302,H317,H319,H335
• pcodes: P261 - P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Sens. 1 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 10 - Combustible liquids
• flash_point_f: 188.6 °F - closed cup
• flash_point_c: 87 °C - closed cup