The calibrator is a specific chemical which is equivalent to 250 U/L alpha-glucosidase. The assay generally yields a delta OD in the range of approximately 0.37 to 0.42 on typical instruments for the calibrator; although values may vary by instrument. The calibrator is stable, and consistent readings indicate proper kit performance. Serum or plasma can be tested directly; if measurements fall outside the kit's detection range, dilution of samples may be necessary.
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MAK123
α-Glucosidase Activity Assay Kit
sufficient for 100 colorimetric tests
Synonym(e):
α-Glucosidase Test Kit
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 kit | Warenkorb auf Verfügbarkeit prüfen | € 574,00 |
Über diesen Artikel
NACRES:
NA.84
UNSPSC Code:
12161503
Technischer Dienst
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Unterstützung erhaltenusage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; neurological disorders
storage temp.
−20°C
Gene Information
human ... GAA(2548)
mouse ... GAA(14387)
rat ... GAA(367562)
General description
α-Glucosidase hydrolyzes carbohydrates by acting on terminal, non-reducing a(1→4)-linked D-glucose residues with the release of D-glucose. Defects in α-glucosidase have been implicated in Pompe disease and diabetes.
Application
Suitable for the detection of α-glucosidase activity in biological samples.
α-Glucosidase Activity Assay Kit has been used to measure the activity of a-glucosidase.
Biochem/physiol Actions
In this assay,α-glucosidase activity is determined by a reaction in which α-glucosidase hydrolyzes p-nitrophenyl-α-D-glucopyranoside resulting in the formation of a colorimetric (405 nm) product, proportional to the α-glucosidase activity present
Features and Benefits
Compatible with high-throughput handling systems.
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Dieser Artikel | |||
|---|---|---|---|
| description sufficient for 100 colorimetric tests | description sufficient for 100 colorimetric tests | description suitable for activity assay, kit of 1 (5 components) | description sufficient for 100 colorimetric tests |
| Gene Information human ... GAA(2548) | Gene Information human ... GBA(2629), GBA2(57704), GBA3(57733) | Gene Information - | Gene Information - |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| usage sufficient for 100 colorimetric tests | usage sufficient for 100 colorimetric tests | usage sufficient for 30 tests (and 10 blanks) | usage - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| relevant disease(s) endocrinological disorders, diabetes; neurological disorders | relevant disease(s) neurological disorders; gastrointestinal diseases; hematological disorder | relevant disease(s) - | relevant disease(s) endocrinological disorders; gastrointestinal diseases; genitourinary disorders |
Lagerklasse
10 - Combustible liquids
Was ist los?
WGK 2
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Does the Calibrator have a predetermined value, or a range for a valid test? Also, is there a target to which samples should be diluted?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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about cat # MAK123, in the the protocol, the samples are incubated either at room temperature or 37°C and then absorbance is measured after 20 min. Please indicate me the incubation time at RT and incubation time at 37C because kinetic is different at T
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The calibrator is incubated under the same conditions as the sample, and the activity is calculated relative to the absorbance of the calibrator, so it doesn't matter what temperature is used for incubation.
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Hi, I have a question about cat # MAK123, a-glucosidase activity assay kit, which is sufficient for 100 assays. Once the reagents are opened, can they be used at a later date? eg - am I able to open the kit and run samples over several days?
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The component included in this kit may be refrozen for later use until the expiration date. Do not store refrigerated.
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