M6908

Myeloperoxidase aus Humanleukocyten

lyophilized powder, ≥50 units/mg protein

• Synonym(e): MPO, Peroxidase, myelo
• CAS-Nummer: 9003-99-0
• NACRES: NA.32
• UNSPSC Code: 12352200
• MDL number: MFCD00131641
• EG-Nummer: 1.11.2.2 (BRENDA, IUBMB)
• eCl@ss: 32160410

Eigenschaften

• biological source: human leucocytes
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥50 units/mg protein
• solubility: H₂O: soluble, aqueous buffers such as 50 mM sodium acetate: soluble (Freezing of the enzyme in solution may result in a substantial loss of activity.)
• UniProt accession no.: P05164
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [O+H2]O[O-]
• InChI: 1S/H2O3/c1-3-2/h1-2H
• InChI key: JSPLKZUTYZBBKA-UHFFFAOYSA-N
• Gene Information: human ... MPO(4353)

Beschreibung

General description

Myeloperoxidase (MPO) is encoded by the gene mapped to human chromosome 17q22-24. The encoded protein is a leukocyte-derived enzyme and is an integral constituent of the innate immune response. MPO belongs to the heme peroxidase superfamily. MPO is a heme protein containing iron and is highly expressed in leukocytes, neutrophils, monocytes and some subtypes of tissue macrophages.[1]

Application

Myeloperoxidase from human leukocytes has been used:

• as a standard to quantify the neutrophil (polymorphonuclear cell [PMN]) infiltration in lung tissue
• as a neutrophil extracelullar trap (NET) component to stimulate 10⁶ dendritic cells or 10⁶ macrophages
• as a standard in myeloperoxidase (MPO) assay to measure pancreatic MPO

Myeloperoxidase (MPO) from human leukocytes has been used in MPO assay.

Biochem/physiol Actions

Myeloperoxidase (MPO) has a crucial role to play in destruction of various microorganisms and foreign cells, such as bacteria, fungi, viruses, red cells and malignant and nonmalignant nucleated cells. MPO catalyzes the production of number of reactive oxidant species (ROS) that influence tissue damage during inflammation. MPO and its downstream inflammatory pathways function as a potent therapeutic target for prophylaxis of atherosclerotic cardiovascular disease. MPO catalyze the synthesis of polychlorinated dibenzo(p)dioxins and furans (PCDD/F) from precursor such as chlorophenols in presence of hydrogen peroxide (H₂O₂).

Physical form

Lyophilisiert aus 0.02 M Natriumacetat-Puffer, pH 6.0

Analysis Note

Das Ausgangsmaterial wurde getestet und reagierte negativ auf Antikörper gegen HIV und HBSAG.

Other Notes

1 U produziert einen Anstieg der Absorption (A₄₇₀) von 1.0 pro Minute bei pH 7.0 und 25 °C, errechnet aus der Anfangsreaktionsgeschwindigkeit unter Verwendung von Guaiacol als Substrat. Gesamtreaktionsvolumen: 3.035 mL.

Lysosomales Protein, das Häm enthält.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable