M6190

Monoclonal Anti-MYOD1 antibody produced in mouse

clone 5.2F, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Anti-Myogenic Differentiation Antigen 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01323984
• Conjugate: unconjugated
• Clone: 5.2F, monoclonal
• Application: ICC, IHC (f), IHC (p), IP, WB
• Citations: 8

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5.2F, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 34 kDa
• species reactivity: human, rat, chicken, mouse
• concentration: 1.0 mg/mL
• technique(s): immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL, immunohistochemistry (frozen sections): 2-4 μg/mL, immunoprecipitation (IP): 2 μg using 1 mg protein lysate, western blot: 1 μg/mL (reacts with the ~45 kDa protein)
• isotype: IgG2a
• UniProt accession no.: P15172
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MYOD1(4654) ; mouse ... Myod1(17927) ; rat ... Myod1(337868)

Beschreibung

General description

Myogenic differentiation antigen 1 (MYOD1) is a nuclear protein which is expressed in skeletal muscles. It is part of the basic helix-loop-helix (bHLH) family of transcription factors. The gene encoding it is localized on human chromosome 11.

Immunogen

recombinant mouse MyoD1 protein.

Application

Monoclonal Anti-MYOD1 antibody produced in mouse has been used in:

• immunofluorescence staining at a 1:50 dilution
• western blotting
• immunostaining at a 1:300 dilution

Biochem/physiol Actions

Myogenic differentiation antigen 1 (MYOD1) maybe involved in recruitment of enzymes like acetyltransferases and methyltransferases to myogenic enhancers in the human genome. It takes part in the regeneration of muscles and mediates muscle cell differentiation by activating cell cycle arrest.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable