L9277

Anti-Leukemia Inhibitory Factor antibody produced in goat

IgG fraction of antiserum, lyophilized powder

• Synonym(e): Anti-LIF
• UNSPSC Code: 51111800
• NACRES: NA.41
• MDL number: MFCD00212999
• Conjugate: unconjugated
• Clone: polyclonal
• Application: Neutral
• Citations: 8

Eigenschaften

• biological source: goat
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): neutralization: suitable
• UniProt accession no.: P15018
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... LIF(3976)

Beschreibung

General description

Leukemia inhibitory factor (LIF) is a pleotrophic glycoprotein that belongs to the IL6 family of cytokines. It is secreted by many cell types such as, fibroblasts, macrophages, T cells, monocytes, liver cells, osteoblasts and cells of endometrium. LIF interacts with a heterodimeric receptor composed of two subunits, gp130 and gp190. A variety of signal transduction pathways are triggered in response to LIF including ERK/MAPK, JAK1, PI3K, STAT3, Jun-cFos and NF-κB. The various functions of LIF include proliferation of adult human T cells, maintenance of pluripotency of embryonic stem cells and inhibition of leukemic ell differentiation. Reports also indicate the important role of LIF in lactation, tissue regeneration after brain and spinal cord injury, muscle regeneration and motor neuron functions.
Anti-Leukemia Inhibitory Factor recognizes human leukemia inhibitory factor. It shows no cross-reactivity with recombinant mouse LIF.

Immunogen

recombinant human LIF expressed in E. coli

Application

Anti-Leukemia Inhibitory Factor antibody may be for immunoblotting a concentration of 1 μg/ml For ELISA a working concentration of 1 μg/ml is recommended. The antibody is suitable for neutralization reactions. For neutralization of LIF in SEK1, melanoma cell line, a concentration of 100 μg/ml was used.

Biochem/physiol Actions

The antibody neutralizes the biological activity of recombinant human LIF.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Preparation Note

Purified using protein G

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)