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Merck

L8629

Sigma-Aldrich

Lektin aus Phaseolus vulgaris (rote Kidneybohne)

Erythroagglutinin PHA-E

Synonym(e):

Phaseolus vulgaris Agglutinin, PHA

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5 MG
€ 221,00

€ 221,00


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5 MG
€ 221,00

About This Item

UNSPSC-Code:
12352202
NACRES:
NA.32

€ 221,00


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Qualitätsniveau

Form

salt-free, lyophilized powder

Wirksamkeit

<10 μg per mL agglutination activity (with erythryocytes)

Zusammensetzung

Protein, ≥80% biuret

Lagertemp.

2-8°C

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Biochem./physiol. Wirkung

PHA besteht aus zwei Molekularspezies, einem Erythroagglutinin (PHA-E) mit niedriger mitogener und hoher erythroagglutinierender Aktivität sowie einem Leucoagglutinin (PHA-L) mit hoher mitogener und hoher leucoagglutinierender Aktivität, aber sehr niedriger erythroagglutinierender Aktivität.
PHA-E ist nicht blutgruppenspezifisch, die Agglutination kann aber durch bestimmte Oligosaccharide gehemmt werden. PHA-P ist die Proteinform von PHA vor Trennung und Aufreinigung von Erythroagglutinin und Leucoagglutinin. PHA-M ist die Mucoproteinform. Konjugate werden aus den entsprechenden aufgereinigten Lektinen zubereitet.

Qualität

Tested for leucoagglutination.

Angaben zur Herstellung

Purified by a modification of the method of Leavitt.[1]

Hinweis zur Analyse

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Tuo Yang et al.
Cell reports, 29(9), 2917-2928 (2019-11-28)
Increased tolerance of Plasmodium falciparum to front-line artemisinin antimalarials (ARTs) is associated with mutations in Kelch13 (K13), although the precise role of K13 remains unclear. Here, we show that K13 mutations result in decreased expression of this protein, while mislocalization
R D Leavitt et al.
The Journal of biological chemistry, 252(9), 2961-2966 (1977-05-10)
Affinity-purified phytohemagglutinin from red kidney bean resolves into five isolectins by SP-Sephadex ion exchange chromatography. Recoveries ranging from 30 to 130 mg of protein for each isolectin are easily achieved. The isolectins have similar amino acid compositions which differ only
Malvina Prapa et al.
NPJ precision oncology, 5(1), 93-93 (2021-10-29)
Glioblastoma is the most malignant primary brain tumor and is still in need of effective medical treatment. We isolated patient-derived glioblastoma cells showing high GD2 antigen expression representing a potential target for CAR T strategy. Data highlighted a robust GD2
H J Hoffmann et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 33(7), 930-935 (2003-07-16)
Atopy is closely associated with the cellular T helper type-2 (Th2) phenotype, that is dominated by the pleiotrophic cytokine IL-4. The cellular source of IL-4 has yet to be determined, although basophils have been proposed. Eosinophils and mast cells are

Artikel

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Questions

1–2 of 2 Questions  
  1. Which PHA is more suitable for cell stimulation and intracellular cytokine staining assays?

    1 answer
    1. Catalog numbers L1668 and L8902 are suitable for cell culture assays and cell stimulation. The end user needs to design and optimize the assays. These products have not been specifically tested for intracellular cytokine staining assays. It is recommended to review the literature for further information.

      Helpful?

  2. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 answer
    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

      Helpful?

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