Catalog numbers L1668 and L8902 are suitable for cell culture assays and cell stimulation. The end user needs to design and optimize the assays. These products have not been specifically tested for intracellular cytokine staining assays. It is recommended to review the literature for further information.
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
5 mg | Warenkorb auf Verfügbarkeit prüfen | € 231,00 |
Über diesen Artikel
€ 231,00
Quality Segment
form
salt-free, lyophilized powder
potency
<10 μg per mL agglutination activity (with erythryocytes)
composition
Protein, ≥80% biuret
storage temp.
2-8°C
Biochem/physiol Actions
PHA-E ist nicht blutgruppenspezifisch, die Agglutination kann aber durch bestimmte Oligosaccharide gehemmt werden. PHA-P ist die Proteinform von PHA vor Trennung und Aufreinigung von Erythroagglutinin und Leucoagglutinin. PHA-M ist die Mucoproteinform. Konjugate werden aus den entsprechenden aufgereinigten Lektinen zubereitet.
Preparation Note
Analysis Note
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| form salt-free, lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 300 |
| composition Protein, ≥80% biuret | composition Protein, 50-70% biuret | composition Protein, ≥60% biuret | composition - |
| potency <10 μg per mL agglutination activity (with erythryocytes) | potency <40 μg per mL agglutination activity | potency erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.), ≤1.0 μg/mL leucoagglutination activity, ≤5 μg/mL mitogenic activity | potency ≤62.5 μg per mL agglutination activity, ≤10 μg/mL mitogenic activity |
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Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Which PHA is more suitable for cell stimulation and intracellular cytokine staining assays?
1 answer-
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What do the designations -P, -L, -M, and -E represent in the names of PHA products?
1 answer-
Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:
1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.
2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.
PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.
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