Direkt zum Inhalt
Merck

Wichtige Dokumente

Gesamtdokumentation anzeigen

Fortfahren mit

L3038

Lysenin from Eisenia foetida

solid

Synonym(e):

Eisenia Lysenin, Lysenin Protein

Anmelden zur Ansicht der Organisations- und Vertragspreise.

Über diesen Artikel

UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
MFCD02687941

form

solid

Quality Segment

200

mol wt

33 kDa

concentration

≥50% (SDS-PAGE)

storage temp.

−20°C

General description

Lysenin is a superfamily of certain proteins including lysenin-related protein 1 (LRP-1, lysenin 2) and LRP-2 (lysenin 3).

Application

Lysenin from Eisenia foetida has been used to treat B cells and study lysenin′s effect on membrane diacylglycerol (DAG) and surface sphingomyelin (SM).

Biochem/physiol Actions

Lysenin is a 33kDa protein present in the coelomic fluid of the earthworm Eisenia foetida. It interacts with sphingomyelin in cell membranes. In vertebrates, this binding results in cytotoxicity and contraction of smooth muscle in vitro as well as vasodepressor activity and lethality under in vivo conditions.
Lysenin serves as a tool to explore membrane lipid organization. It is known to induce hemolysis in vertebrates and mammalian cells.

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumente section.

Wenn Sie Hilfe benötigen, wenden Sie sich bitte an Kundensupport

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Questions

  1. What's the solubility of the Lysenin samples in water? I desovate the 50ug sample in 0.6 mL water, looks a lot of not solutable? How to you usaually desovate the samples? In water or any buffers? I am woring on Lysenin reconstitution in to lipid bilyers.

    1 answer

    1. The solubility for the product is not analyzed on a lot to lot basis. However, it has been cited that solution were prepared by dissolving lysenin (1 mg/ml in 2% BSA-PBS) or 50 μg of lysenin in 50 μl of 10 mM HEPES/KOH (pH 7.0) containing 30 mM KCl and 50% Glycerol.

      Kindly review the following citations:
      Yamaji A, Sekizawa Y, Emoto K, Sakuraba H, Inoue K, Kobayashi H, Umeda M. Lysenin, a novel sphingomyelin-specific binding protein. J Biol Chem. 1998 Feb 27;273(9):5300-6. doi: 10.1074/jbc.273.9.5300. PMID: 9478988.

      Faouri RA, Krueger E, Govind Kumar V, Fologea D, Straub D, Alismail H, Alfaori Q, Kight A, Ray J, Henry R, Moradi M, Salamo G. An Effective Electric Dipole Model for Voltage-induced Gating Mechanism of Lysenin. Sci Rep. 2019 Aug 7;9(1):11440. doi: 10.1038/s41598-019-47725-0. PMID: 31391571; PMCID: PMC6686002.

      Helpful?

Reviews

No rating value

Active Filters